How does it work? Ca2+-Imaging method to examine CNG-channels
1) The cells are loaded with a Ca2+-sensitive fluorescence-dye (Fluo-4,AM)
2) Then, the CNG-channels are activated/opened by the cyclic nucleotide
3) This opening of the CNG-channels causes Ca2+-influx into the cells
→ The cells now show a fluorescence, that can easily be detected.